Identification of putative baroreceptors in human aortic arch by histological and omics analyses

Figures

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  Fig. 1. Optimised immunohistochemical staining of nerves in the human aortic arch (n = 3). Different antibodies were used against various types of peripheral nerves. VGLUT1 and 2 vesicular glutamate transporter 1 and 2 (dilution 1:1000 and 1:75), TH tyrosine hydroxylase (dilution 1:1000), NF neurofilament (dilution 1:300), BDNF brain-derived neurotrophic factor (dilution 1:500), MBP myelin basic protein (dilution 1:300), PGP9.5 protein gene product 9.5 (dilution 1:200), CGRP calcitonin gene-related peptide (dilution 1:500)

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  Fig. 2. Selected immunohistochemical staining of nerves of various sizes in the human aortic arch. Antibodies against tyrosine hydroxylase (TH, 1:1000)) neurofilament (NF, 1:300)) and vesicular glutamate transporter 2 (VGLUT2, 1:75) on consecutive slides were used. Scale bars 50 µm

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  Fig. 3. Quantification of nerve distribution along the aortic arch using tyrosine hydroxylase (TH) staining. A Example of the image quantification. The blue line indicates the separation between the outer and inner curvature. The nerves (TH-positive staining) are indicated by red lines. B Quantification plot showing the number of nerves in the individual segments (see Supplementary Fig. 1). C Quantification plot focusing on the TH-stained area. D A schematic image of the aortic arch with the individual segments analysed in the study. The blue dashed line displays the part of the aortic arch with the most nerves and nerve endings

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  Fig. 4. Proteomics. A Proteome analysis - Diagram showing the total number of proteins present in the LCM samples and the number of proteins found only in the group with nerves and without nerves. B Number of proteins detected in the various experimental approaches: LCM (laser capture microdissection), FFPE (macrodissection from formalin-fixed in paraffin-embedded samples), and Fresh (fresh frozen tissue samples). C Proteome analysis - raw protein abundance in the adventitia of the FFPE aortic tissue samples. PIEZO1 piezo-type mechanosensitive ion channel 1, TRPV2 and TRPM4 transient receptor potential ion channels V2 and M4, AoA aortic arch, hAo healthy abdominal aorta, AAA abdominal aortic aneurysm. The table shows the mean ± standard deviation of the individual detected putative mechanosensitive ion channels