bated for 16-18 hr. with borate buffer, pH 10, in the presence of n-butanol; purification by calcium phosphate gel at alkaline and acid pH; repeated precipitations of the enzyme with half-saturated ammonium sulphate; and separation from inactive proteins by dialysis. 2. From 1 kg. ox kidneys it is possible to obtain 620 mg. of a preparation with a Q02 value not less than 700.
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Norman et al. (1953) studied this question.
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