William J. Bemrick, Byron L. Buchli, Henry J. Griffiths, Development of Dirofilaria immitis in Anopheles quadrimaculatus after Exposure of the Microfilariae to a Freezing Temperature, The Journal of Parasitology, Vol. 51, No. 6 (Dec., 1965), pp. 954-957
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Bemrick et al. (1965) studied this question.
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