Metabolic profiles of MK-3984 (SARM) in zebrafish using ultra high performance liquid chromatography Q extractive HF hybrid quadrupole orbitrap mass spectrometry (UPLC-QE-HF-MS) for doping control

-acetylated-cysteinylated metabolites in negative ion mode. Three key metabolites-M1 (reductive defluorinated metabolite), M28 (reductive defluorinated, monohydroxylated and sulfated metabolite), and M55 (reductive defluorinated, monohydroxylated and cysteinylated metabolite) were identified as potential biomarkers for monitoring MK-3984 abuse. These findings provide valuable insights into the metabolic fate of MK-3984 and establish a foundation for its detection in anti-doping controls.