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When an ethylene dichloride solution of enniatin A is shaken with potassium picrate in potassium hydroxide solution, picrate anion is extracted into the organic phase in amount proportional to the enniatin concentration. Enniatin A can thus be determined spectrophotometrically in the range 5-60 μg/ml. Neutral lipids do not interfere. Valinomycin can be determined similarly, but cyclodepsipeptides that lack cationophoric properties are not estimated in this method.
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Audhya et al. (1973) studied this question.
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