What question did this study set out to answer?

To determine whether ischemic neuronal damage in the vestibular end organs is linked to changes in cellular glutamate concentrations.

April 12, 2012Acta Oto-Laryngologica

Immunoelectron microscopic analysis of neurotoxic effect of glutamate in the vestibular end organs during ischemia

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Authors

ASAkira SasakiHirosaki University AMAtsushi MatsubaraHirosaki University KTKeiji TabuchiApple (Israel)

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Implication

Experimental study reveals time-dependent glutamate release and nerve damage in guinea pig inner ears during ischemia, indicating excitotoxicity drives vestibular injury after prolonged blood loss.

Key Points

To determine whether ischemic neuronal damage in the vestibular end organs is linked to changes in cellular glutamate concentrations.
Induced transient local anoxia (10 or 30 minutes) in guinea pig inner ears by compressing the left labyrinthine artery, using the contralateral right ears as controls.
Evaluated morphological damage and quantified areal gold particle densities representing glutamate in vestibular end organs using post-embedding immunoelectron microscopy.
Areal gold particle densities in type I and type II hair cells and supporting cells were noticeably lower in the ischemic ear compared to controls, reflecting glutamate release.
Tissues subjected to 10 minutes of ischemia showed no remarkable morphological alterations compared to controls.
Tissues subjected to 30 minutes of ischemia exhibited swollen bouton-type endings and significantly enlarged intercellular spaces between type I hair cells and nerve chalices.

Cite This Study

Sasaki et al. (2012) studied this question.

synapsesocial.com/papers/6a8cfd293b974ccba12f09ac https://doi.org/10.3109/00016489.2012.656322

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Implication

Experimental study reveals time-dependent glutamate release and nerve damage in guinea pig inner ears during ischemia, indicating excitotoxicity drives vestibular injury after prolonged blood loss.

Immunoelectron microscopic analysis of neurotoxic effect of glutamate in the vestibular end organs during ischemia

Discussion

Member takes

Implication

Key Points

Cite This Study

Immunoelectron microscopic analysis of neurotoxic effect of glutamate in the vestibular end organs during ischemia

Discussion

Member takes

Implication

Key Points

Cite This Study

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