Both putatively native(n) commercial calf thymus DNA and 14C labeled human KB DNA contained significant amounts of denatured DNA (dDNA) as determined by methylated albumin‐kieselguhr chromatography. The proportion of dDNA in these preparations was sufficient to cause confusion regarding the antigenic specificity of anti‐DNA antibody when unfractionated DNA was used in either the counterimmunoelectrophoresis or the Farr assays for anti‐DNA antibody. Serum from patients with systemic lupus erythematosus contained both anti‐nDNA and anti‐dDNA antibodies. About 50% of patients with symptomatic procainamide‐induced lupus (PLE) exhibited anti‐dDNA; none had anti‐nDNA. Procainamide treated patients with a positive ANF who did not have symptomatic PLE had neither anti‐dDNA nor anti‐nDNA.
No takes yet. Share an insight, caveat, or question.
Winfield et al. (1974) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: