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苜蓿银纹夜蛾核多角体病毒(Autographa californica nucleopolyhedrovirus, AcMNPV)基因组含有3个细胞凋亡抑制基因, 即p35, iap1和iap2. 其中, p35作为一个有效的依赖于天冬氨酸的半胱氨酸蛋白酶(caspase)抑制因子, 能够抑制多种因素诱发细胞凋亡, 而iap1和iap2的功能仍未完全明晰, 本研究对IAP1和IAP2的功能进行了详细分析. 缺失了p35的AcMNPV仍可抑制棉铃虫核多角体病毒(Helicoverpa armigera single nucleocapsid NPV, HearNPV)诱导的BTI-Tn-5B1-4(Tn-Hi5)细胞凋亡并挽救HearNPV在Tn-Hi5细胞中复制及HearNPV出芽型病毒粒子的产生. 进一步构建了瞬时表达质粒以及分别表达AcMNPV的p35, iap1和iap2基因的重组HearNPV, 转染瞬时表达的IAP1和IAP2对HearNPV感染诱导的Tn-Hi5细胞凋亡有抑制效果, 而重组病毒感染Tn-Hi5细胞也可抑制其凋亡并在其中复制, 然而重组HearNPV表达的p35, iap1和iap2并未能挽救出芽型病毒粒子的产生. 结果表明, AcMNPV的iap1和iap2基因表达产物作为细胞凋亡抑制因子是有功能的.
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