Reflections upon Sperm–Endosalpingeal and Sperm–Zona Pellucida Interactions In Vivo and In Vitro

In terms of experimental studies of the process of fertilization in mammals, this essay attempts to give a balanced assessment of current interpretations and to raise questions of direct relevance to two fields of research. First, the nature of pre-ovulatory sperm-endosalpingeal binding is set in a physiological perspective, and examination of this dynamic process in vitro is shown to suffer from serious shortcomings. In particular, sperm-epithelial binding reactions demonstrated in cultures of endosalpingeal cells may represent only a portion of the binding reaction in vivo or, as revealed by sperm binding to the tracheal epithelium, could be largely non-specific. Second, concerning the subsequent phase of binding of a fertilizing spermatozoon to the zona pellucida, the nature of the initial contact arrest of the sperm head is evaluated, especially in the light of the highly uneven or 'pitted' surface of this outer coating of the oocyte. Bearing in mind the active phase of oviduct macromolecular secretion that continues after ovulation, the question is posed as to how functional sperm binding sites are preserved on the surface of the zona pellucida rather than masked by epithelial and cumulus cell secretions. Finally, it is recalled that initial sperm : egg ratios at the site of fertilization in the ampullary-isthmic region of the oviduct are close to unity in diverse species of mammal, such fertilizing spermatozoa being released from prominent reserves in the caudal isthmus shortly before the time of ovulation. On strictly quantitative grounds, therefore, the extent to which biochemical or molecular studies portray changes in the vanguard of fertilizing spermatozoa needs to be questioned. Such studies are more likely to be descriptive of overall changes in very large numbers of sperm cells at diverse stages of maturation.