Lactic dehydrogenase and cytochrome b2 of baker's yeast. The deoxyribose polynucleotide component and the physicochemical properties of the crystalline enzyme

with 0-12 ml. of 10u-H2S04 and 0-05 ml. of 72% (v/v) perchloric acid, the digest was diluted to 1 5 ml. with water, hydrolysed for 10 min. at 1000 and cooled. The inorganic phosphate was then estimated, acid being omitted from the molybdate reagent.