Push–pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand–receptor interactions

), high fluorescence quantum yields and red/far-red operating spectral range. It also displays much higher photostability in apolar media as compared to Nile Red, a fluorogenic dye of similar color. Its reactive carboxy derivative has been successfully grafted to carbetocin, a ligand of the oxytocin G protein-coupled receptor. This conjugate exhibits >1000-fold turn on between apolar 1,4-dioxane and water. It targets specifically the oxytocin receptor at the cell surface, which enables receptor imaging with excellent signal-to-background ratio (>130). We believe that presented push-pull dioxaborine dye opens a new page in the development of fluorogenic probes for bioimaging applications.