Emulsification of Liposomes with Incomplete Freund's Adjuvant: Stability of the Liposomes and the Emulsion

Emulsification of liposomes with incomplete Freund's adjuvant, a water-in-oil emulsion, resulted in the formation of stable emulsions containing a large fraction of intact liposomes. Although some loss of liposome integrity and loss of emulsion stability did occur at certain concentrations of liposomes, based on the release of trapped glucose, it was determined that formulations of Freund's adjuvant containing liposomes could be produced that still retained a considerable liposomal permeability barrier for at least 7 days.