On Splenic Lymph and Its Hemolytic Action

The method of collecting the splenic lymph of dogs is explained, by which the flow and protein content are measured.2. The flow volume of the splenic lymph is very small and ranges from 0.002 cc. to 0.009 cc. per minute.3. The protein content is relatively higher, ranking to next the liver lymph, corresponding to 52-72 per cent of serum. A greater quantity of hemoglobin is contained in the splenic lymph even when collected under the best conditions.4. Time required for dyes to pass from the blood into the splenic lymph is increased with an increase in the molecular weight of the substance. The dye concentration in the splenic lymph ranks under that of the liver lymph.5. A hemolytic substance of high activity is contained in the splenic lymph. Hemolytic actitivity is inhibited by hemoglobin and accelerated by incubation (at 56°C, for 30 minutes). The lymph dervied from other sources contained a hemolytic substance, whose activity is lower than that of the splenic lymph.