Among the many influencing factors which govern the nephelometric value (N.V.) of fatty acids and cholesterol, I wish to discuss, in the present paper, certain important ones in their relation to saponification, because practically all quantitative fat determinations necessitate such a procedure.These factors consist of, on the one hand, substances which are essential to the determination, such as the reagents, or those which facilitate the production of colloidal suspensions, designated as protective colloids; and, on the other hand, conditions like heat, concentration, and the sequence of mixing the reagents in the development of turbidity.However, before we apply either a procedure like saponification, or employ a protective colloid, we must ascertain its influence on the nephelometric values of the substances in question; an increase in the value is always to be desired, but a decrease is liable to render the determination less delicate, and therefore unsatisfactory as a micro method.Studies of this type are of interest not only as a preliminary step to the development of a new method, but also as a means of discovering the defects or proving the accuracy of a method already in use.In the first paper,l where the suggested term nephelometric vahie was defined, the importance of acid concentration upon the nephelometric values of the fatty ingredients was emphasized and it was shown that fatty acids, as well as cholesterol, have specific nephelometric values.To overcome the difhculties caused by the fact that there exists a difference between the 1 Csonka, F.
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F.A. Csonka (1920) studied this question.