An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme

First reported allosyltransferase: The allopyranosyl analogue of sucrose (All-Fru) was used as donor for the glucansucrase GTFA from Lactobacillus reuteri 121. This enzyme is able to hydrolyse All-Fru and transfer allose to acceptor substrates such as sugars, amino acids and (−)-epicatechin, thus forming artificial α-linked allopyranosyl products. A triple GTFA mutant showed twofold increase in allosyl transfer.