In this review, methods of Agrobacterium T-DNA transfer into plant cells in planta are discussed. The main focus is on the technologies of gene transfer into generative plant cells as a part of Agrobacterium T-DNA. The influence of the plant genotype, bacterial strain, vector construction type, inoculation medium composition, and the conditions of plant treatment with Agrobacterium on the efficiency of Agrobacterium transformation in planta is analysed. Based on literature and personal experimental data, the possible mechanism of Agrobacterium transformation of generative plant cells in planta is discussed.
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Chumakov et al. (2012) studied this question.
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