Further Demonstration of Bacterial Lipids in Mycobacterium Bovis Harvested From Infected Mouse Lungs

Mycobacterium bovis grown in vivo were separated by differential centrifugation from homogenate of the experimentally infected mouse lungs and subjected to lipid fractionation by Anderson-Lederer method in comparison with the same strain grown on Sauton synthetie liquid medium. The per cent yield of chloroform-soluble wax was much smaller than that of wax A in the lung bacilli. The esters of mycocerosic acid were the main components of wax A of the in vivo bacilli, but some esters of mycolic acid were not detected unlike the case of the Sauton bacilli. An arabinolipid, probably arabinose mycolate, and tuberculostearic acid-containing cardiolipin were separated from bound lipids and the phospholipid fraction, respectively, as the lipids common in both kinds of bacilli.