Effect of decreased temperature on the tissue dissolution ability of sodium hypochlorite: An in vitro study

Purpose:The present in vitro study aimed to compare the tissue dissolution ability of a 5% sodium hypochlorite solution at 3 different temperatures.Methods: Thirty standardized fragments were prepared from bovine muscle tissue and randomly divided into 3 experimental groups (n = 10) according to the temperature of the sodium hypochlorite.The tissue was immersed in a 1.5-mL test tube containing 20 mL of sodium hypochlorite at the specific temperature and stored for 15 min.The solution was agitated with an ultrasonic tip working for 1 min.Then the solution was filtered, and the tissue sample was dried.The weight loss of the tissue was measured as dissolved tissue by the sodium hypochlorite.A one-way analysis of variance was used to compare the mean dissolved tissue weight between groups (p < 0.05). Results:The highest dissolution values were found in the 60°C sodium hypochlorite group, achieving significantly greater mass loss (p < 0.05), while no significant difference was found between the solutions applied at 20°C and 2.5°C (p > 0.05). Conclusion:This in vitro study found that the application of sodium hypochlorite by cooling for cryotherapy did not alter its capacity for dissolving organic tissue compared to application at room temperature.