Antimicrobial studies of stem bark extract and their phytoconstituent from Semecarpus anacardium L

Background & Objective: To investigate the antimicrobial properties of stem bark extract of S. anacardium andtheir phytoconstituent using agar well diffusion and in silico docking methods. Methodology: In vitro antibacterialactivity of petroleum ether, chloroform, methanol stem bark extract of S. anacardium was screened against both gramnegative and gram positive bacteria. Results: The methanol extract of stem bark and amentoflavone, a bioactivecompound showed significant antibacterial activity against Klebsiella pneumonia and antifungal activity againstCandida albicans. In silico docking of amentoflavone with bacterial glucosamine-6-phosphate synthase and threefungal targets, mevalonate-5-diphosphate decarboxylase (1FI4), Sec3 - Rho1 complexes (3A58), Kre2p/Mnt1pa1, 2-mannosyltransferase (1S4N) showed significant inhibition with minimum binding energy, when compare to standarddrug ciprofloxacin and amphotericin B. Conclusion: This study clearly showed that the amentoflavone used as broadspectrum of antimicrobial drug