The preparation of highly purified arginase solutions (Bach & Killip, 1958, 1961) proved some- what laborious. Further work led to a much shorter and easily reproducible method by the use of which an extract of arginase from acetone-dried powder of ox liver could be purified to the precrystallization stage in 2 days of laboratory work. MATERIALS L( + )-Arginine hydrochloride was obtained from the Sigma Chemical Co., St Louis, Mo., U.S.A. The Arlco jackbean meal used in the preparation of urease was supplied by Hopkin and Williams Ltd., Chadwell Heath, Essex. The 'manganese-maleate solution' used in the prepa- ration of arginase was made by dissolving 8-9 g. of MnSO4,4H20 and 1-8 g. of disodium maleate in glassdistilled water and making the volume up to 1 1.
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Bach et al. (1963) studied this question.