Efficacy of Type 2 PRRSV vaccine against challenge with the Chinese lineage 1 (NADC30-like) PRRSVs in pigs

Figures

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  Figure 1. Phylogenetic tree based on the ORF5 genes of the 2 isolates and reference viruses. Reliability of the tree was assessed by bootstrap analysis of 1000 replications. Our representative isolates were marked with the black cicrle (•).

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  Figure 2. Recombination between the JXA1 P45 and NADC30 was detected in FJWQ16 by means of RDP (A) and boot scanning. (B) One recombination break points were detected at positions 6854–8787 of the sequence alignment (with reference to VR-2332 strain).

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  Figure 3. Comparison of mean rectal temperatures (±S.D.) among vaccinated, non-vaccinated and control groups: (A) FJZ03 challenge groups; (B) FJWQ16 challenge groups. The data were expressed as the mean ± S.D. of the numbers of pigs alive at the time of the sample collection.

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  Figure 4. Survival rates of piglets after challenge with FJWQ16 (A) or FJZ03 (B).

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  Figure 5. Mean respiratory score from pigs in vaccinated, non-vaccinated and control groups. Different letter superscripts denote significant differences at p < 0.05.

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  Figure 6. Fold of total body weight gain among vaccinated, non-vaccinated and control groups was calculated by considering the weight of the pig on day 0 as 1. Different letter superscripts denote significant differences at p < 0.05.

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  Figure 7. Mean values of the genomic copy number of PRRSV RNA in serum samples. The data were expressed as the mean ± S.D. of the numbers of pigs alive at the time of the sample collection.

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  Figure 8. Serum neutralizing antibodies were detected using PRRSV infection of Marc-145 cells. Asterisk denotes that the NA titer of MLV + FJZ03 challenge group had a significantly higher than other groups (p < 0.05). The error bars represent standard deviations of the three experiments.