Key result
Exogenous application of gangliosides reduced the cross-linking efficiency of GH-DAF from 73% to 49%, indicating displacement of GPI-anchored proteins from lipid microdomains in living cells.
Absolute Event Rate: 49% vs 73%
Exogenous application of gangliosides displaces GPI-anchored proteins from sphingolipid-cholesterol microdomains in living cells, providing a potential mechanism for their effects on cellular signal transduction.
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May alter signal transduction via membrane displacement; leaves open in vivo human relevance.
Simons et al. (1999) studied this question. Exogenous gangliosides (GM1 or bovine brain gangliosides) vs. Untreated cells or NBD-C6-HPC was evaluated on Cross-linking efficiency of GH-DAF. Exogenous application of gangliosides reduced the cross-linking efficiency of GH-DAF from 73% to 49%, indicating displacement of GPI-anchored proteins from lipid microdomains in living cells.
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