Combined Effect of Salt Concentration and Redox Potential of the Medium on the Initiation of Vegetative Growth by Clostridium welchii

Summary Four strains of Clostridium welchii grew in a medium containing 6% (w/v) of NaCl within 14 days at 37°. In one instance growth was obtained with 7% of NaCl from a large, but not a small, inoculum. Three of the strains grew from a large inoculum in the presence of 0.5 % of NaCl at Eh values of + 194 to + 238 mv. In a medium containing 5% of NaCl no growth occurred unless the Eh level was considerably reduced. The fourth strain was less affected until the NaCl concentration was increased to 6%.