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The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH. DNAs from three of the six non-diazotrophic strains were also found to possess such homology.
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Oakley et al. (1988) studied this question.
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