SUMMARY: Spheroplast suspensions containing as few as one rod in a thousand were made with Escherichia coli (15 his -) by the use of penicillin. These spheroplasts could be reverted to the rod state with efficiency approaching 100%. Spontaneous mutation from histidine requirement to non-requirement was observed in these spheroplasts, in which cell division did not take place.
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Ryan et al. (1963) studied this question.
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