Conditions for the production of Agrocin 84 byAgrobacterium radiobacter K84

A chemically defined medium was developed that supported the growth ofAgrobacterium radiobacter K84 and the production of agrocin 84. Various supplements were investigated for their effect on growth rate and production of agrocin 84 using a well-diffusion assay method. Mannitol was found to be a better substrate for growth ofA. radiobacter K84 compared to the other sugar alcohols and sugars tested. By contrast,d-fructose was the better substrate for the production of agrocin 84. Biotin supplementation stimulated production of agrocin 84 but did not eliminate the diauxic lag seen with the basal medium. The opine, octopine, inhibited growth ofA. radiobacter K84 and production of agrocin 84 as did coenzyme B12. By contrast, the cytokinin, isopentenyl adenosine, was marginally stimulatory to production, as was vitamin B12. Acetosyringone supplementation had a negligible effect on growth rate and production of agrocin 84.