Key result
Treatment-naïve dogs with multicentric lymphoma had significantly lower median serum clusterin concentrations (58.34 ug/ml) compared to age-matched healthy controls (98.93 ug/ml).
Why the study?
Clusterin expression is associated with human lymphomas, but serum clusterin had not been assessed as a biomarker in canine lymphoma.
Case-Control (n=90)
No
Absolute Event Rate: 58.34% vs 98.93%
p-value: p=<0.001
Serum clusterin levels are significantly lower in dogs with multicentric lymphoma compared to healthy controls, though wide individual variation may limit its utility as a single diagnostic biomarker.
Lower clusterin may aid lymphoma detection in dogs; leaves open biomarker validation in prospective studies.
Clusterin (CLU), also known as apolipoprotein J, is a widely expressed, heterodimeric, glycoprotein, important in tumourigenesis, apoptosis and immunoregulation. In humans, CLU expression has been associated with anaplastic large cell and Hodgkin's lymphoma. In this study, serum CLU levels in dogs with multicentric lymphoma (MLSA) were compared with healthy control dogs, using both western blot and enzyme-linked immunosorbent assay (ELISA). Western blot confirmed the presence of CLU in dog sera at the predicted molecular weight and the relative levels detected correlated with the levels detected by ELISA. CLU level analysis by ELISA found treatment naïve dogs with MLSA had a significantly (P < .001) lower serum CLU level compared with healthy controls. However, there was no significant difference between MLSA dogs prior to treatment and in complete remission. The wide variation in serum CLU levels may limit its potential as a single candidate biomarker for MLSA, although any prognostic predictive value of serum CLU concentrations has yet to be assessed.
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McNaught et al. (2019) conducted a case-control in Canine multicentric lymphoma (MLSA) (n=90). Multicentric lymphoma (MLSA) vs. Healthy controls was evaluated on Serum clusterin concentration (p=<0.001). Treatment-naïve dogs with multicentric lymphoma had significantly lower median serum clusterin concentrations (58.34 ug/ml) compared to age-matched healthy controls (98.93 ug/ml).
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