Microglia in the pineal gland of 1-day-old Sprague-Dawley rats were examined by OX-42 immunocytochemistry and DiI-acetylated-LDL uptake in pineal cell suspension and were found to comprise 3-5% of the total cells in the pineal gland of the neonates. In order to investigate the effects of microglia on pinealocyte structure and function, microglia-depleted and microglia-enriched pineal cell cultures were generated from 1-day-old neonate by fluorescence activated cell sorting (FACS). After 7 days of culture, tissues were processed for either immunocytochemistry for pinealocyte S-antigen and serotonin or high performance liquid chromatography to measure serotonin. Morphometric analysis of immunoreacted cells revealed that pinealocyte neurite length was enhanced in microglia-depleted cultures and was inhibited in a microglia-enriched environment (ANOVA, P < 0.001). Serotonin content of pineal cultures decreased in microglia-depleted cultures and was elevated in microglia-enriched cultures (ANOVA, P < 0.001) without any significant change in pinealocyte numbers. These findings are consistent with a working hypothesis that microglia function to mediate neuroendocrine-immune interactions of the gland.
No takes yet. Share an insight, caveat, or question.
Tsai et al. (1997) studied this question.
Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context: