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Abstract—HPLC analysis of neurosporene extracted from the chromatophore membranes ofRhodobacter spheroidesG1C showed two isomeric components 1 and 2. Extract from the light‐harvesting complex (LH) gave only component 2, while extract from the reaction center (RC) mainly gave component 1. Both components were isolated, and their configurations were determined by means of (1) resonance Raman, (2) electronic absorption, and (3)1H‐HMR spectroscopy. The configuration of component 2 originating from LH was determined to be all‐transand that of component 1 from RC was determined to be 15‐cis. Thus, our previous configurational prediction of this particular carotenoid bound to RC, by means of resonance Raman spectroscopy, to be15‐cis[Y. Koyama, T. Takii, K. Saiki and K. Tsukida,Photobiochem. Photobiophys.5(1983)139–150] is confirmed.
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Koyama et al. (1988) studied this question.
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