Electrophoresis and Assay of Trypsin Inhibitors in Different Stages of Tempe Production

Tempe was made according to a laboratory method giving the Indonesian product. At several stages samples were taken, trypsin inhibitors were extracted with different procedures (high pH, salt) and purified with affinity chromatography. A standard assay method for trypsin inhibitors showed large differences in the activity of the extracts. The purified samples were subjected to isoelectric focusing and sodium dodecyl sulfate-electrophoresis, with which mainly Kunitz inhibitors were found. Utilisation of 2-mercaptoethanol in sodium dodecyl sulfate-electrophoresis proved that Kunitz inhibitors had been modified by affinity chromatography.