The Isolation and Structure Identification of Uridine 5-(2-Acetamido-2-deoxy-α-D-glucopyranosyluronic Acid Pyrophosphate) from Micrococcus lysodeikticus Cells

The chemical structure of the major component of the nucleotides isolated from Micrococcus lysodeikticus cells was established as being that of uridine 5-(2-acetamido-2-deoxy-α-d-glucopyranosyluronic acid pyrophosphate) by comparison of the products of hydrolysis of the nucleotide and of its reduced derivative with authentic 2-amino-2-deoxy-d-glucuronic acid and with 2-acetamido-2-deoxy-d-glucose, respectively. The base was identified by examination of the ultraviolet spectra at various wave lengths; the anomeric configuration of the amino sugar residue was established by comparison of the optical rotation with that of other sugar nucleotides; and the ring configuration of the amino sugar residue was determined by periodate oxidation to give glyceric acid.