Summary Of the total amount of staphylococcal a-toxin produced in batch cultures, 1 per cent. or less appeared to be cell-associated, indicating rapid release of the toxin into the environment. The level of cell-associated haemolysin reached its maximum at the onset of the stationary phase of growth and decreased thereafter. Cell-associated haemolysin was isolated by the combined use of gel filtration and iso-electric focusing and was characterised by several criteria. Purified material closely resembled the extracellular form of staphylococcal a-toxin. The authors gratefully acknowledge the photographic assistance of Mr Ian McKee. This work was supported in part by grants from The Royal Society and the Scottish Home and Health Department.
No takes yet. Share an insight, caveat, or question.
McNiven et al. (1972) studied this question.