The Distribution of Labelled Aldosterone in Mice Using Whole Body Autoradiography

Whole body autoradiography of mice 2, 20 and 60 minutes after intravenous injection of 14C‐4‐aldosterone showed a rapid and high accumulation of aldosterone and/or its metabolites in the liver, bile and kidney indicating the routes of excretion. Besides the uptake in the kidney and intestine, well known as target organs for aldosterone, a high uptake was also registered in ductus deferens, caput epididymidis and adrenal cortex. Thin‐layer chromatography of chloroform extract of selected tissues showed high amounts of unchanged aldosterone 2 minutes after the injection, but after 20 minutes scintillation counting showed that most of the radioactivity was found in the water phase and/or bound to the tissue residues.