Two‐Step Production of Oil Enriched in Conjugated Linoleic Acids and Diacylglycerol

A two‐step process was used to produce diacylglycerol‐enriched structured lipid that contained mainly c9,t11 and t10,c12 isomers of conjugated linoleic acids (CLA). First, a structured triacylglycerol (TAG) was synthesized by lipase‐catalyzed acidolysis of corn oil with CLA. This structured triacylglycerol contained 30.4 mol% CLA with 45.5% of the CLA mostly located at sn‐1,3 positions of the glycerol backbone. Then, lipase‐catalyzed glycerolysis was conducted between structured triacylglycerol and glycerol to produce diacylglycerol‐enriched structured lipid. The final product contained 6.8% monoacylglycerol, 31.5% diacylglycerol and 61.1% TAG after 48 h reaction. The selected chemical (fatty acid composition, the content of mono‐, di‐, and triacylglycerol in the reaction product) and physical properties (melting profile) were determined by hihg‐performance liquid chromatography (HPLC), gas chromatography (GC), and differential scanning calorimetry (DSC).