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July 25, 2022Open Access

Diagnostic reliability of serum active vitamin B12 (holo-transcobalamin) in true evaluation of vitamin B12 deficiency:Relevance in current perspective

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Why the study?

Measurement of total vitamin B12 raised concerns over early detection of deficiency due to clinical unreliability, prompting an assessment of active vitamin B12 efficacy.

Does active vitamin B12 measurement improve diagnostic accuracy for true vitamin B12 deficiency compared to total vitamin B12 in patients referred for testing?

Population

100 participants referred for vit B12 assay

Comparison

Active vit B12 vs total vit B12

Design

Retrospective study

Key result

Active vitamin B12 demonstrated higher sensitivity (82.35% vs 65%) and specificity (46.6% vs 43.8%) compared to total vitamin B12 for diagnosing true vitamin B12 deficiency.

Authors

RDRinini DastidarKSKunal Sikder

Discussion

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Overview

Active B12 may aid deficiency detection in referred patients; leaves open need for prospective outcome validation before routine adoption.

Study Design

Type

Observational (n=100)

Multicenter

No

Structured PICO

Does active vitamin B12 measurement improve diagnostic accuracy for true vitamin B12 deficiency compared to total vitamin B12 in patients referred for testing?

P
Population
100 participants referred for vitamin B12 assay at a single center in India to evaluate the diagnostic accuracy of active versus total vitamin B12.
E
Exposure
Measurement of serum active vitamin B12 (holo-transcobalamin)
C
Comparator
Measurement of serum total vitamin B12
O
Outcome
Diagnostic sensitivity and specificity for true vitamin B12 deficiencysurrogate

Main Result

Absolute Event Rate: 82.35% vs 65%

Active vitamin B12 (holo-transcobalamin) is a more sensitive and specific biomarker than total vitamin B12 for the early and accurate diagnosis of vitamin B12 deficiency.

Limitations

  • Limited sample size
  • Limited population type without accounting for racial and ethnic variation
  • Inability to test methylmalonic acid (MMA) due to financial restraints
  • Limited population type lacking racial and ethnic variation

Cite This Study

Dastidar et al. (2022) conducted an observational in Vitamin B12 deficiency (n=100). Active vitamin B12 (holo-transcobalamin) assay vs. Total vitamin B12 assay was evaluated on Sensitivity for true vitamin B12 deficiency. Active vitamin B12 demonstrated higher sensitivity (82.35% vs 65%) and specificity (46.6% vs 43.8%) compared to total vitamin B12 for diagnosing true vitamin B12 deficiency.

synapsesocial.com/papers/6a902eb0e16d749e8fc91874https://doi.org/10.21203/rs.3.rs-1802865/v1
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