Answers are grounded in this paper and cite their sources.
β-Glucuronidase (β-D-glucuronide glucuronohydrolase, EC 3.2.1.31) from liver lysosomes of male Sprague–Dawley rats was purified by (NH4)2SO4 precipitation, treatment with trypsin and gel filtration on Sephadex G-200, and then fractionated into five active components by ion-exchange chromatography on DEAE-Sephadex A-25 or DEAE-cellulose. All five isoenzymes were shown to be electrophoretically and ultracentrifugally homogeneous. Their sedimentation coefficients range from 12.27 to 13.39. All five isoenzymes appear to be glycoproteins.
No takes yet. Share an insight, caveat, or question.
Potier et al. (1973) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: