Gram Reaction in Crushed Yeasts

Thick smears of yeasts are dried, crushed between slides or cover-slips, stained with anilin crystal violet 11/2 minute, Lugol's iodine solution 2 minutes, decolorized in undiluted anilin oil, and counterstained with 1% aqueous safranin. Photomicrographs are given of crushed yeasts, thus stained, which show a Gram-positive outer layer and a Gram-negative inner substance.