Investigation into an outbreak of Border disease virus in pigs in England

Figures

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  FIGURE 1. Histopathology of formalin‐fixed tissues stained with haematoxylin and eosin (H&E). (a) Kidney with segmental to global membranous glomerulonephritis (in five out of six pigs). (b and c) Liver and lymph node, respectively, with marked extramedullary haematopoiesis (in four out of six pigs). Bar = 100 μm

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  FIGURE 2. Representative images of BDV immunohistochemistry of the brain. Intense neuronal labelling (arrows) was particularly observed in lacunar layer of hippocampus together with smaller numbers of glial cells, morphology suggestive of astrocytes, mostly in hippocampus (a). Rare labelling of a similar population of cells was also seen in cerebral cortex (b). Similar pattern of immunostaining was observed in the brain of other two piglets examined from this submission

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  FIGURE 3. Phylogenetic analysis of partial 5′ UTR sequence of porcine Border disease viruses (BDV). The evolutionary analyses were conducted in MEGA X (Kumar et al., 2018) using the Maximum Likelihood method and Tamura–Nei model (Tamura & Nei, 1993). The tree with the highest log likelihood (−9353.20) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Representative BDV strains of the different sub‐genotypes are included in the tree and denoted by GenBank accession number, strain or isolate name, the animal species of origin and the country of isolation. BVDV‐1 NADL isolate was used as outgroup. The partial 5′ UTR sequences is corresponding to nucleotides 136 to 358 (223 nucleotides) of reference strain X818 (accession number NC_003679.1)