The N-Terminal Mutations of cMyBP-C Affect Calcium Regulation, Kinetics, and Force of Muscle Contraction

Figures

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  Figure 1. Normalized calcium dependences of slow muscle fiber tension (a) and stiffness (b) with WT, P161S, and D75N fragments of cMyBP-C. Symbols show experimental points, and lines are their approximation by the Hill Equation (1). Experimental points are shown as mean ± SD.

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  Figure 2. Tension redevelopment (lower panel) in muscle fiber after a run of Protocol 1 at pCa 5.5 (solid lines) and pCa 6.8 (dashed lines) in the control and in the presence of the WT C0-C2 fragment of cMyBP-C. The left tension scale relates to pCa 5.5, and the right one is for pCa 6.8. The upper panel shows changes in the sarcomere length ΔSL in nm per half-sarcomere.

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  Figure 3. Example of the tension transient in the muscle fiber on the run of Protocol 2 in the control (black line) and in the presence of the P161S C0-C2 fragment (red line). The upper panel shows changes in sarcomere length in nm per half-sarcomere.

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  Figure 4. Typical tension response of a slow fiber to a release-stretch protocol at the saturating calcium concentration (pCa 5.5). Changes in sarcomere length ΔSL in nm per half-sarcomere are shown in the upper panel. The tension response is fitted by an exponential function (red dots).