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December 1, 1991Applied and Environmental MicrobiologyOpen Access

Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus

Laurent Benbadis J R Garel Donna L. Hartley

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Abstract

SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.

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Benbadis et al. (1991) studied this question.

synapsesocial.com/papers/6a9055ca3dd9003d79c913a0 https://doi.org/10.1128/aem.57.12.3677-3678.1991

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