Development of an Electrochemical Biosensor for the Rapid Detection of Cholera Toxin Using Air Stable Lipid Films with incorporated Ganglioside GM1

The present work describes the investigations of electrochemical interactions of cholera toxin with stabilized lipid films supported on a methacrylate polymer on a glass fiber filter with incorporated ganglioside GM1 for the development of a biosensor for cholera toxin. The analyte was injected into the flowing streams of a carrier electrolyte solution, the flow of the solution stops for 5 min and an ion current transient was obtained, the magnitude of which is correlated to the toxin concentration with detection limits of 0.06 µM. The method was applied in blood serum samples. Further work is directed to the rapid detection of other toxins used in bioterrorism using this novel ultrathin film technology.