In Vitro Propagation of Borage

In vitro shoot proliferation of borage ( Borago officinalis L.) was achieved in a basal medium based on Murashige and Skoog salts supplemented with 17.6 μ m BA (4 mg·liter −1 ) plus 10% (v/v) coconut water. Rooting of in vitro-produced shoots occurred in basal media and increased in response to the addition of IBA. Rooted shoots were transferred successfully to soil. Immature zygotic embryos cultured in 4.5 μ m 2,4-D (1 mg·liter −1 ) plus coconut water (CW) produce asexual embryos directly from the cotyledonary surface and indirectly from callus. Chemical names used: N -(phenylmethyl)-1 H -purin-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D); 1 H -indole-3-butanoic acid (IBA).