Some Studies on the Identification of Rumen Bacteria with Fluorescent Antibodies

SUMMARY: Fluorescent antisera to a number of isolates of rumen bacteria have been prepared and used to demonstrate the presence of these organisms in situ in rumen contents. The serological tests for a specific organism in rumen contents of a number of calves agree with the isolations of this organism from the same sources. A mieroseope suitable for this work, the preparation and purification of antisera and the preparation of specimens is described.