White Leghorn pullets were fed standard diets supplemented with 0.0%, 0.1%, 0.5%, and 1.0% levels of dietary caffeine. The two highest levels of caffeine caused the production of soft-shelled eggs and a cessation of egg production within 3 days. Although 0.1% caffeine did not effect fertility, it significantly increased embryonic mortality over a 14-day period following a single insemination.
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Ax et al. (1974) studied this question.
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