Answers are grounded in this paper and cite their sources.
Colonies of Clostridium botulinum could be easily distinguished from meat particles by supplementing Wynne agar with 0.4% egg yolk. The pour-plate method was suitable for enumeration of C. botulinum, provided the medium was covered with a layer of agar containing 0.01% dithiothreitol. Viable counts of heat-treated spores were consistently higher in Wynne agar supplemented with egg yolk (Wynne-EY agar) than in Wynne agar alone.
No takes yet. Share an insight, caveat, or question.
Hauschild et al. (1977) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: