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Linoleic acid from soybean oil was used to synthesize conjugated linoleic acid (CLA), and the response surface methodology (RSM) was applied to optimize the process. A temperature of −35 °C and a solvent to oil sample ratio of 8 were suggested for removal of saturated fatty acids by low-temperature crystallization. The ratio of oil sample/urea/methanol suggested was 1:2:5.5 (w/w/v) for removal of oleic acid by urea crystallization. A temperature of 150 °C and a time of 140 min were found to be the optimal conditions in the isomerization for the production of c-9,t-11 and t-10,c-12 CLA isomers.
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Yang et al. (2004) studied this question.
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