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Incubation of β-MSH with extracts of brain leads to disappearance of the melanophoretic activity. This effect occurs as a logarithmic function of incubation time, is temperature and pH dependant and seems therefore to be due to an enzymatic inactivation. This effect was not observed in incubating β-MSH with extracts of liver or muscle similarly prepared.
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Long et al. (1961) studied this question.
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