KB-R7943 reduces 4-aminopyridine-induced epileptiform activity in adult rats after neuronal damage induced by neonatal monosodium glutamate treatment

Figures

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  Fig. 1. Neonatal MSG treatment effects on NCX1-3 protein expression in the entorhinal cortex (left) and hippocampus (right) at PD60. Upper panels a and c correspond to representative images of western blots for each analysed protein in control (CTL) and MSG-treated rats (MSG). Lower panels b and d correspond to graphic representations of the relative expression of each NCX protein with respect to β-actin expression. The colourless bars correspond to the CTL group, and the dark bar to the MSG group. Values represent the mean ± SDM from five animal samples for each cerebral region and group analysed in triplicate. Statistically different at *p < 0.05 and **p < 0.01 for the MSG compared to the CTL group using a Student’s t-test

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  Fig. 2. EEG activity generated in the entorhinal cortex and hippocampus of control rats (CTL group) at PD60 by i.c.v. infusion of KB-R7943 at different doses. Representative one-minute fragments from the recordings taken during basal activity (5 min before the infusion), at the onset of altered activity, and 1 h after the infusion was completed are presented for 500, 250, 125 and 62.5 pmol of KB-R7943

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  Fig. 3. EEG activity generated in the entorhinal cortex and hippocampus of control rats (CTL group) at PD60 with i.c.v. infusion of 4-AP (3 nmol), KB-R7943 (62.5 pmol) or the combination of 4-AP (3 nmol) and KB-R7943 (62.5 pmol). Representative one-minute fragments from the recordings taken during basal activity (5 min before the infusion), at the onset of altered activity, and 30 and 60 min after completion of the infusion are presented

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  Fig. 4. EEG activity generated in the entorhinal cortex and hippocampus of MSG-treated rats at PD60 with i.c.v. infusion of 4-AP (3 nmol), KB-R7943 (62.5 pmol) or the combination of 4-AP (3 nmol) and KB-R7943 (62.5 pmol). Representative one-minute fragments from the recordings taken during basal activity (5 min before the infusion), at the onset of altered activity, and 30 and 60 min after completion of the infusion are presented. KB-R7943 significantly reduced epileptiform activity induced by 4-AP in MSG-treated rats

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  Fig. 5. KB-R7943 (62.5 pmol) effects on epileptiform EEG activity induced by 4-AP (3 nmol) in control (colourless bars) and MSG-treated (black bars) rats. The latency, duration and net amplitude of the first burst recorded in the right hippocampus after i.c.v. infusion of the drugs alone or in combination are presented in panel a, b and c, respectively. Furthermore, the duration of ictal activity d and elapsed time before EEG activity normalization e were also measured in the same conditions described before. Activity recorded in the right hippocampus was considered for these measurements because it was the region closest to the infusion location. Data represent the mean ± SEM from four animals for each infusion scheme (subgroups: 4-AP, KB-R7943, and 4-AP + KB-R7943) in each group (CTL and MSG). Statistically different at *p < 0.05 for all subgroups compared to that in control rats with 4-AP; @p < 0.05 for all subgroups compared to that in MSG-treated rats with 4-AP; &p < 0.05 for all subgroups compared to that in control rats with KB-R7943; #p < 0.05 for all subgroups compared to that in control rats with 4-AP + KB-R7943; and §p < 0.05 between KB-R7943 alone and 4-AP + KB-R7943 in…