Transcription of the Vibrio cholerae haemolysin gene, hlyA, and cloning of a positive regulatory locus, hlyU

Transcription of the Vibrio cholerae hlyA gene, which encodes a cytotoxic haemolysin, has been investigated. The hlyA transcript initiates 430 nucleotides (nt) upstream of the translational start site. hlyA-cat transcriptional fusion constructs were active in V. cholerae but not in Escherichia coli. An hlyA-cat fusion was used to select, from a V. cholerae O17 plasmid library, a clone that could activate the hlyA promoter in E. coli. This regulatory locus has been designated hlyU. hlyU appears to be distinct from the previously described hlyR locus.