Simultaneous Determination of Captopril and Statins in API, Pharmaceutical Formulations and in Human Serum by RP‐HPLC

Co‐administration of statins with angiotensin converting enzyme inhibitors is most common, since there is strong synergy between hypertension and hypercholesterolemia in terms of risk factors for the development of cardiovascular diseases. Rapid liquid chromatographic procedure proposed for analysis of captopril and statins in pharmaceutical preparations and human serum using acetonitrile:water (60:40 v/v) as a mobile phase, adjusting pH to 2.9 with UV detection at 230 nm. The advantages of this method include good and rapid separation, well resolved peaks, and only a small amount of sample is required for assay and adequate precision. The method showed good linearity in the range of 2.5‐100 μgmL−1 for captopril and 0.625‐25 μgmL−1 for statins with a correlation coefficient of 0.9999. The recovery of captopril and statins (rosuvastatin, atorvastatin and simvastatin) was > 99.9% and > 99.3%, respectively. The proposed method may be used for the quantitative analysis of captopril and statins alone or in combination in bulk drugs, dosage formulations and in human serum.